Create your config.yaml file for ProHap Peptide Annotator

General parameters






Input file

Input format
Column containing the unique identifier for the PSM / peptide. This has to be unique per row.
Column containing the amino acid peptide sequence - if modifications, etc. are included, they will be ignored, and we consider I = L (e.g., 'M.n[+42.021]PEPTIDEK2.A' = 'PEPTLDEK').
(?) Column containing the list of protein accessions matching the concatenated FASTA file, separated by semicolon (e.g., 'prot_123ab;prot_234cd'). If left blank, the protein IDs will be inferred from the FASTA file - note that this is computationally expensive and will take time.
Column containing the positions of the first amino acid within each of the proteins listed in the protein column, indexed from 0, separated by semicolon. If left blank, the peptide positions will be inferred from the FASTA file.
Column containing the label distinguishing target and decoy peptides. Leave this field blank if decoys are not reported.
Value of the label denoting a decoy peptide (e.g., "1" or "decoy"). Leave blank if decoys are not reported.
Separator

Output file

Check that no fields below are labbeled as MISSING and or copy the content to your config.yaml file: